Composite

Part:BBa_K552002

Designed by: Jonathan Herrmann   Group: iGEM11_WashU   (2011-09-27)


CrtYB Gene-Codon Optimized for Yeast

This DNA sequence codes for the binary enzyme CrtYB. CrtY (Lycopene Beta-cyclase) converts lycopene into beta-carotene and serves as the last step in beta-carotene biosynthesis in Saccharomyces cerevisiae. CrtB is acts further upstream of CrtY in the pathway and is called Phytoene Synthase. CrtB converts geranylgeranyl diphosphate to phytoene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1239
    Illegal XbaI site found at 1305
    Illegal XbaI site found at 1545
    Illegal XbaI site found at 1917
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 723
    Illegal BglII site found at 1308
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1239
    Illegal XbaI site found at 1305
    Illegal XbaI site found at 1545
    Illegal XbaI site found at 1917
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1239
    Illegal XbaI site found at 1305
    Illegal XbaI site found at 1545
    Illegal XbaI site found at 1917
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
Parameters
None